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plants-user@lists.vbcf.ac.at

December 2020

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New soil available for testing - Klasmann Substrate 2

by Jez,Jakub

Dear all, as you know, we had some troubles with the soil (Gramoflor 2006) und subsequent plant stress symptoms (anthocyanin accumulation in leaves). It turned out that this was mainly caused by the lack of nutrients, especially of phosphorous, (details please see mail below). The last weeks we did successfully test a very promising substrate: “Klasmann – Deilmann Substrat 2” with excellent references (e.g. ETH Zürich and PSI HT phenotyping platforms). This substrate comes with a very balanced composition, structure/texture and nutrient mix. But it does not only look good on paper; our tests with Arabidopsis (Col-0 and numerous accessions/mutants; thx Tesi, Schlögelhofer group) showed that no stress symptoms appeared (under LED and fluo-tube light). Furthermore, it does not require sieving before potting. This Klasmann Substrate 2 is now available for you at the soil room for testing! Your feedback is of great importance and very much appreciated! We are available as usual for your questions, comments and complaints. Best regards, yours PlantS Team. [VBC Logo] Jakub Jez Head, Plant Sciences Facility T +43 1 7962324 7090<tel:+43179623247090> M +43 664 8084 77090<tel:+43%20664%208084%2077090> jakub.jez(a)vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at> Vienna BioCenter Core Facilities GmbH Dr. Bohr-Gasse 7 1030 Vienna Austria www.vbcf.ac.at<https://www.vbcf.ac.at> Chair, Austrian Plant Phenotyping Network (APPN<https://www.appn.at/>) and Support Group Member of ESFRI EMPHASIS<https://emphasis.plant-phenotyping.eu/> project Twitter: @VBCF_PlantS<https://twitter.com/VBCF_PlantS> LinkedIn: www.linkedin.com/in/jezjakub<http://www.linkedin.com/in/jezjakub> [A close up of a tree Description automatically generated] FFG PHENOPlant<https://www.viennabiocenter.org/projects-grants/phenoplant/>: Austrias 1st multi-sensor, high-throughput plant phenotyping infrastructure launching 2021! From: Jakub Jez <jakub.jez(a)vbcf.ac.at> Date: Thursday, 28. November 2019 at 11:03 To: GMI All <gmi.all.intern(a)gmi.oeaw.ac.at>, VBCF Pant Sciences Facility user mailing list <plants-user(a)lists.vbcf.ac.at> Cc: "VBCFplants.GRP" <plants(a)vbcf.ac.at> Subject: PLANT STRESS NEWS! Please read! Dear friends, In the last weeks/months we have been investigating the plant stress issue, mainly focusing on the LED light spectrum. Always comparing to the fluorescent tube standard. Surprisingly, also the fluorescent tube setup shows same plant stress issues! Which was not the case in the very beginning when we have tested and decided for the new type of soil. See picture attached to this mail: Phytotron: #21 Soil: Gramoflor2006; not sieved; with perlite Light: Fluorescent tubes; LD Watering: Flooding twice a week Left side of the tray: WUXAL treated plants (once, after 1 month, 2ml/L) Right side of the tray: untreated plants Our new hypothesis focuses on the soil. In particular, on the soil composition and the available nutrients. From today’s point of view, it looks like the composition of the soil might have changed over the last months (without informing us); but this needs further investigation. The automated watering system (flooding) also eventually contributes negatively to the problem by washing out nutrients; twice a week. Also sieving might be suboptimal. Red leaves are known to be a symptom for Phosphor/Phosphate deficiency. We are in touch with the soil supplier and with APPN soil scientist from the BOKU to find a long-term solution. As a quick fix * you can fertilize your plants with WUXAL fertilizer which is available in the Facility (once after ~1month; 2ml/L; watering from the top). * Use a long-term fertilizer (OSMOCOTE) * Use the old type of soil ED63 Please contact Anneliese in case of questions for the quick fix. We will keep you posted on further steps and present you soon our final results and the long-term solution. Looking forward to your valuable feedback on your quick-fix results. Best wishes, yours PlantS Team [VBC Logo] Jakub Jez Head, Plant Sciences Facility T +43 1 7962324 7090<tel:+43179623247090> M +43 664 8084 77090<tel:+43%20664%208084%2077090> jakub.jez(a)vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at> Vienna BioCenter Core Facilities GmbH Dr. Bohr-Gasse 3 1030 Vienna Austria www.vbcf.ac.at<https://www.vbcf.ac.at> Chair, Austrian Plant Phenotyping Network (APPN<https://www.appn.at/>) and Support Group Member of ESFRI EMPHASIS<https://emphasis.plant-phenotyping.eu/> project Twitter: @VBCF_PlantS<https://twitter.com/VBCF_PlantS> LinkedIn: www.linkedin.com/in/jezjakub<http://www.linkedin.com/in/jezjakub> [signature_949803173] FFG PHENOPlant<https://www.ffg.at/sites/default/files/allgemeine_downloads/strukturprogram…>: Austrias 1st multi-sensor, high-throughput plant phenotyping infrastructure launching 2021!

Dec. 16, 2020