Dear Peter, Dear all, Thank you for your input; I appreciate the open discussion. That basically confirms our findings and the literature: There is a soil/nutrient AND a light component that is causing the stress; but the one is probably linked to the other. Please refer to the publications and references listed in our last slide. Our hypothesis also considers both factors; but what it further proposes is, that if we fix the soil/nutrient issue the light factor has no, or at least a not significant, effect (still under deeper investigation but proofed by our first experiment). Do you agree? And again, the most stressed plants in our experiment were grown under fluorescent tubes. There was not one stressed plant at any light conditions in all the fertilized samples. Your experiment proofs the high variability of results under suboptimal soil/nutrient conditions (remember the “jumping bad-guy and good guy” game). And YES; the LED light is designed to be closer to the natural sunlight and is therefore most probably more “powerful” than the artificial fluorescent tube spectrum; the more we should take care on the on the factors. Important remark to the experiment setup in respect to this and future experiments: Based on the images in your presentation (slide 11 to 14) it is very difficult, or even impossible to evaluate or judge, because of the different light conditions that were used for imaging! One either needs to take all the samples out of the phytotron to uniform light conditions for imaging (like e.g.we did, ring-light is available at our Facility), or use a color standard (available in our Facility) and correct for the light in a post-process manner. Otherwise the green color of the plant will always appear different (good/bad) in different setting although it is same colored; even the tray per se looks stressed under LED light (bluish/reddish). Therefore, we are happy to collaborate and be more involved in future experiments. More tests (by PlantS) are planned for 2020 and we will keep you up to date; we will also arrange a user meeting February/March. Looking forward to your feedback. Merry x-mas and a healthy and successful year 2020 to all of you! Best wishes, Jakub and the PlantS team. [VBC Logo] Jakub Jez Head, Plant Sciences Facility T +43 1 7962324 7090<tel:+43179623247090> M +43 664 8084 77090<tel:+43%20664%208084%2077090> jakub.jez@vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at> Vienna BioCenter Core Facilities GmbH Dr. Bohr-Gasse 3 1030 Vienna Austria www.vbcf.ac.at<https://www.vbcf.ac.at> Chair, Austrian Plant Phenotyping Network (APPN<https://www.appn.at/>) and Support Group Member of ESFRI EMPHASIS<https://emphasis.plant-phenotyping.eu/> project Twitter: @VBCF_PlantS<https://twitter.com/VBCF_PlantS> LinkedIn: www.linkedin.com/in/jezjakub<http://www.linkedin.com/in/jezjakub> [signature_1073383129] FFG PHENOPlant<https://www.ffg.at/sites/default/files/allgemeine_downloads/strukturprogramme/FuE-Infrastrukturf%C3%B6rderung/Kurzfassung_PHENOPlant.pdf>: Austrias 1st multi-sensor, high-throughput plant phenotyping infrastructure launching 2021! From: Peter Schloegelhofer <peter.schloegelhofer@univie.ac.at> Date: Monday, 23. December 2019 at 16:04 To: VBCF Pant Sciences Facility user mailing list <plants-user@lists.vbcf.ac.at> Cc: GMI All <gmi.all.intern@gmi.oeaw.ac.at>, "Soroldoni,Daniele" <daniele.soroldoni@vbcf.ac.at>, "VBCFplants.GRP" <plants@vbcf.ac.at> Subject: PLANT STRESS - some news form the Schlögelhofer Lab Dear All, in addition to the VBCF tests sent by Jakub the other day (many thanks!) we also have some interesting additions to make. Please refer to the attached pptx. As you will see, when using the soil from Gramoflor without any additional fertiliser but with fluorescent tubes, plants are perfectly fine during their life-time. The same soil, without fertiliser, leads to severe stress symptoms under LED lights. It is important to note, that plants grown on this non-fertilised soil and fluorescent tubes but exposed to stray light from LEDs develop stress symptoms (not so severe as under direct LED light, but still). This latter point also helps to straighten some ambiguity with respect to some earlier tests made with fluorescent tubes. In this sense, we need to consider two stress factors: light emitted by the LED lamps and sub-optimal soil. The pptx also contains some pictures testing the former ED63 soil and taller pots. We will now evaluate if the "double saved plants” (fertilised soil and fluorescent tubes) perform better for us (meiosis!) than the “single saved plants” (fertilised soil and LEDs). Best regards, Peter p.s.: have a great season break and a smooth transition to 2020! On 10 Dec 2019, at 17:20, Jez,Jakub <jakub.jez@vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at>> wrote: Dear all, please find the presentation of the resent results attached to this mail. Nine different spectral conditions, including the fluorescent tube control, were tested and treated (fertilized). Details of the experiment design are listed in the presentation. High-resolution pictures/presentation is available for download here (to allow to zoom in): https://www.dropbox.com/s/jqyp1dsny7c0hmh/plant_stress_investigation_1012201... A short summary: Irrespective of the spectral conditions, most of the untreated plants show, to a certain extent, stress symptoms and Anthocyanin accumulation. This applies also to the fluorescent tube control spectrum (!). In this particular experiment the fluorescent tube control shows even the strongest stress symptoms. On the other hand, ALL treated (fertilized) samples look very healthy and do not show any stress symptoms. This quick test and the literature listed seems to proof our hypothesis: “Photooxidative stress caused by nutrient (Pi?) deficiency”. We will continue with the investigation focusing on the soil and nutrients. A new type of soil should arrive soon for testing (details see presentation). Please contact me in case of any questions, comments or complains. Best wishes, yours PlantS Team! From: Jakub Jez <jakub.jez@vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at>> Date: Thursday, 28. November 2019 at 11:03 To: GMI All <gmi.all.intern@gmi.oeaw.ac.at<mailto:gmi.all.intern@gmi.oeaw.ac.at>>, VBCF Pant Sciences Facility user mailing list <plants-user@lists.vbcf.ac.at<mailto:plants-user@lists.vbcf.ac.at>> Cc: "VBCFplants.GRP" <plants@vbcf.ac.at<mailto:plants@vbcf.ac.at>> Subject: PLANT STRESS NEWS! Please read! Dear friends, In the last weeks/months we have been investigating the plant stress issue, mainly focusing on the LED light spectrum. Always comparing to the fluorescent tube standard. Surprisingly, also the fluorescent tube setup shows same plant stress issues! Which was not the case in the very beginning when we have tested and decided for the new type of soil. See picture attached to this mail: Phytotron: #21 Soil: Gramoflor2006; not sieved; with perlite Light: Fluorescent tubes; LD Watering: Flooding twice a week Left side of the tray: WUXAL treated plants (once, after 1 month, 2ml/L) Right side of the tray: untreated plants Our new hypothesis focuses on the soil. In particular, on the soil composition and the available nutrients. From today’s point of view, it looks like the composition of the soil might have changed over the last months (without informing us); but this needs further investigation. The automated watering system (flooding) also eventually contributes negatively to the problem by washing out nutrients; twice a week. Also sieving might be suboptimal. Red leaves are known to be a symptom for Phosphor/Phosphate deficiency. We are in touch with the soil supplier and with APPN soil scientist from the BOKU to find a long-term solution. As a quick fix * you can fertilize your plants with WUXAL fertilizer which is available in the Facility (once after ~1month; 2ml/L; watering from the top). * Use a long-term fertilizer (OSMOCOTE) * Use the old type of soil ED63 Please contact Anneliese in case of questions for the quick fix. We will keep you posted on further steps and present you soon our final results and the long-term solution. Looking forward to your valuable feedback on your quick-fix results. Best wishes, yours PlantS Team <image001.png> Jakub Jez Head, Plant Sciences Facility T +43 1 7962324 7090<tel:+43179623247090> M +43 664 8084 77090<tel:+43%20664%208084%2077090> jakub.jez@vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at> Vienna BioCenter Core Facilities GmbH Dr. Bohr-Gasse 3 1030 Vienna Austria www.vbcf.ac.at<https://www.vbcf.ac.at/> Chair, Austrian Plant Phenotyping Network (APPN<https://www.appn.at/>) and Support Group Member of ESFRI EMPHASIS<https://emphasis.plant-phenotyping.eu/> project Twitter: @VBCF_PlantS<https://twitter.com/VBCF_PlantS> LinkedIn: www.linkedin.com/in/jezjakub<http://www.linkedin.com/in/jezjakub> <image002.png> FFG PHENOPlant<https://www.ffg.at/sites/default/files/allgemeine_downloads/strukturprogramme/FuE-Infrastrukturf%C3%B6rderung/Kurzfassung_PHENOPlant.pdf>: Austrias 1st multi-sensor, high-throughput plant phenotyping infrastructure launching 2021! <plant_stress_investigation_10122019_2.pdf>_______________________________________________ plants-user mailing list plants-user@lists.vbcf.ac.at<mailto:plants-user@lists.vbcf.ac.at> https://www.vbcf.ac.at/mailman/listinfo/plants-user Dr. Peter Schlögelhofer Department of Chromosome Biology Max Perutz Labs University of Vienna Dr. Bohr-Gasse 9 1030 Vienna Austria Phone: +43 1 4277 56240 Email: peter.schloegelhofer@univie.ac.at<mailto:peter.schloegelhofer@univie.ac.at>
