July 2019 - plants-user - lists.vbcf.ac.at

3rd APPN Meeting presentations published!
by Jez,Jakub 12 Jul '19

12 Jul '19

Dear all, We have just published all presentations of the 3rd Meeting and an image gallery! Visit our homepage for download and enjoy the read: https://www.appn.at/3rdappn/ Best regards, Jakub [VBC Logo] Jakub Jez Head, Plant Sciences Facility T +43 1 7962324 7090<tel:+43179623247090> M +43 664 8084 77090<tel:+43%20664%208084%2077090> jakub.jez(a)vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at> Vienna BioCenter Core Facilities GmbH Dr. Bohr-Gasse 3 1030 Vienna Austria www.vbcf.ac.at<https://www.vbcf.ac.at> Chair, Austrian Plant Phenotyping Network (APPN<https://www.appn.at/>) and Support Group Member of ESFRI EMPHASIS<https://emphasis.plant-phenotyping.eu/> project Twitter: @VBCF_PlantS<https://twitter.com/VBCF_PlantS> LinkedIn: www.linkedin.com/in/jezjakub<http://www.linkedin.com/in/jezjakub> [signature_1620261999] FFG PHENOPlant<https://www.ffg.at/sites/default/files/allgemeine_downloads/strukturprogram…>: Austrias 1st multi-sensor, high-throughput plant phenotyping infrastructure launching 2021!

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Hydroponics+Arabidopis=Araponics
by Jez,Jakub 12 Jul '19

12 Jul '19

Dear all, We are currently running an external hydroponics-based phenotyping experiment in phytotron #1. In case you are interested in hydroponics please feel free to take a look through the window and/or talk to Anneliese. Protocols are also available at our facility. Basically, plants are germinating on agar in a sample holder until the roots reach the fluid medium. Advantages are a highly homogenous and controlled root environment; the possibility of working with different chemicals (fertilizers, hormones, salt, etc.) and the root system is easily accessible for sampling or shovelomics (until a certain developmental stage; then roots get knotted). And: no black flies, so far. Disadvantages: handling and potential contaminations of the agar in the sample holder. The system we use is called Araponics: http://www.araponics.com/ Best, Jakub [VBC Logo] Jakub Jez Head, Plant Sciences Facility T +43 1 7962324 7090<tel:+43179623247090> M +43 664 8084 77090<tel:+43%20664%208084%2077090> jakub.jez(a)vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at> Vienna BioCenter Core Facilities GmbH Dr. Bohr-Gasse 3 1030 Vienna Austria www.vbcf.ac.at<https://www.vbcf.ac.at> Chair, Austrian Plant Phenotyping Network (APPN<https://www.appn.at/>) and Support Group Member of ESFRI EMPHASIS<https://emphasis.plant-phenotyping.eu/> project Twitter: @VBCF_PlantS<https://twitter.com/VBCF_PlantS> LinkedIn: www.linkedin.com/in/jezjakub<http://www.linkedin.com/in/jezjakub> [signature_656262654] FFG PHENOPlant<https://www.ffg.at/sites/default/files/allgemeine_downloads/strukturprogram…>: Austrias 1st multi-sensor, high-throughput plant phenotyping infrastructure launching 2021!

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PHENOBox upgrade to 12x12 agar-plates (screening of seedlings and roots)
by Jez,Jakub 04 Jul '19

04 Jul '19

Dear all, Together with Natalie, Philipp and Stephan, our summer students, we are currently implementing and upgrading two PHENOBox systems at both locations of the PlantS facility (GMI and MFPL): https://twitter.com/VBCF_PlantS/status/1146382324604526592 The PHENOBox is a semi-automated imaging cabinet for low-throughput RGB phenotyping of tall plants (up to 50cm incl. pot) and was published last year (Djamei group): https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15129 Now, we are upgrading this system by a sample holder for 12x12cm agar-plates for screening of seedlings and for root phenotyping. Also, subsequent image analysis pipelines will be established. On the top to our samples, we would very much appreciate receiving yours (tall plants, agar-plates with seedlings or roots) in order to have a broad range of different samples for establishing robust image analysis pipelines (please don’t let me dick in the facility waste bins for that ;-). We will also design and build templates for standardized positioning of seeds (seedlings and root phenotyping). Your input (your templates) and experienced is appreciated! If you are interested in this project, please feel free to drop by our office for a chat and a look at the PHENOBox. Thanks! Kind regards, Jakub [VBC Logo] Jakub Jez Head, Plant Sciences Facility T +43 1 7962324 7090<tel:+43179623247090> M +43 664 8084 77090<tel:+43%20664%208084%2077090> jakub.jez(a)vbcf.ac.at<mailto:jakub.jez@vbcf.ac.at> Vienna BioCenter Core Facilities GmbH Dr. Bohr-Gasse 3 1030 Vienna Austria www.vbcf.ac.at<https://www.vbcf.ac.at> Chair, Austrian Plant Phenotyping Network (APPN<https://www.appn.at/>) and Support Group Member of ESFRI EMPHASIS<https://emphasis.plant-phenotyping.eu/> project Twitter: @VBCF_PlantS<https://twitter.com/VBCF_PlantS> LinkedIn: www.linkedin.com/in/jezjakub<http://www.linkedin.com/in/jezjakub> [signature_613215582] FFG PHENOPlant<https://www.ffg.at/sites/default/files/allgemeine_downloads/strukturprogram…>: Austrias 1st multi-sensor, high-throughput plant phenotyping infrastructure launching 2021!

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